Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.036597 |
Chromosome: | chromosome 5 |
Location: | 1499785 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre05.g234655 | (1 of 1) K17550 - protein phosphatase 1 regulatory subunit 7 (PPP1R7, SDS22) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAACCAAGACCGAGGGGGGGTAGAGGGGGCGTAGCCGTTTGTGTGGAGA |
Internal bar code: | TTATAGCTTTGAGTACGATTCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1909 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 9 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCACCAGATACCCACACACA |
Suggested primer 2: | GATCTGTAGACAGGACGGCG |