| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.036610 |
| Chromosome: | chromosome 3 |
| Location: | 8182926 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200400 | (1 of 1) PTHR30383 - PERIPLASMIC BROAD-SPECIFICITY ESTERASE/LIPASE/PROTEASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGTAGCAGTAATACCGCAACACAGCTCGCGAAAAGCCTCCGGCCTCC |
| Internal bar code: | GTATTTATGAATCTTTTGAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2423 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCCTACCCGCATCACTC |
| Suggested primer 2: | TCAGTCGTCTAGGTCCAGCA |