Insertion junction: CLIP2.036612_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.036612
Chromosome:chromosome 3
Location:9033251
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g202673 CSC2 Chlamydomonadale-specific family C protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGAGCTGTGAGTAGGCCGCGCGGCGCCGCGGCTGCAGCAGCACGGGGCG
Internal bar code:TTCGATGAATACGATATGGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:3768
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:48
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:CACACGGGGTCAAACTCGTA
Suggested primer 2:CACCACTGGTTGCACACATG