Insertion junction: CLIP2.036624_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.036624
Chromosome:chromosome 17
Location:4034776
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g728450 (1 of 1) IPR006626//IPR010308//IPR011050 - Parallel beta-helix repeat // TRP-like family // Pectin lyase fold/virulence factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGCAACGTCACACTGGTTGCTGTCCGCGGGCCCAGAAGCCGCATGAAC
Internal bar code:TAATTTCGTGACTATTGTATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:4181
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGAAGACTGAGGCATGC
Suggested primer 2:AACATTCCGCTGACCGAGTT