| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.036648 |
| Chromosome: | chromosome 1 |
| Location: | 5497475 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g039000 | CDS | ||
| Cre01.g039050 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGTGGCAGTGCCGGGGCCAGCAGTGCGCCCATTCTGCGGCCGCAGCT |
| Internal bar code: | TGCAATTATTAAGATGGTTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1770 |
| LEAP-Seq percent confirming: | 23.6842 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGGTCACAACCTCCAGA |
| Suggested primer 2: | CAGAGCCTACACCCGTCATC |