| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.036649 |
| Chromosome: | chromosome 13 |
| Location: | 3724790 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g801564 | (1 of 3) 1.14.13.39 - Nitric-oxide synthase (NADPH) / NO synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGACTGCAGGCCTTGCCACGCCACCCACGAAACTTCCATGCCCACCCAC |
| Internal bar code: | CATCCGATAGTGTACATGCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4957 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCTACACCAAGAACGGA |
| Suggested primer 2: | CGAGCCAAAGTCACACTCCT |