| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.036652 |
| Chromosome: | chromosome 2 |
| Location: | 2388340 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091000 | (1 of 1) PF05346 - Eukaryotic membrane protein family (DUF747) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCGCACGCCTGCCGTCGTAGGGTCACCAAATGAGGCATGGGCGACGGG |
| Internal bar code: | TAACCGAGGGTCTATCATCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1228 |
| LEAP-Seq percent confirming: | 59.6491 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAACCTCAGCAGCGATGTC |
| Suggested primer 2: | GGTGTCTGGGTCTGGACAAG |