Insertion junction: CLIP2.036672_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.036672
Chromosome:chromosome 10
Location:1068671
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g425200 (1 of 1) 1.8.3.5 - Prenylcysteine oxidase / Prenylcysteine lyase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCGTAGTACGCGTACTATGTACTACGTACTGTACTATGTACTGTACGC
Internal bar code:ACCTGTTGAAGGCAGGAGTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1956
LEAP-Seq percent confirming:87.0968
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:TTGCTACCGTGTGTCTCACC
Suggested primer 2:GCGACTGCTACCCACCTATC