| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.036677 |
| Chromosome: | chromosome 6 |
| Location: | 6708862 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g295250 | (1 of 1) K07252 - dolichyldiphosphatase (E3.6.1.43) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTGCGGGTACGGCTGGGCTACCACACGACGCCCCAGGTGCGTGGCTG |
| Internal bar code: | ATGTGACTTTGCCGGGGCCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2492 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 99 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 108 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCATCTACTCCCACGAGC |
| Suggested primer 2: | CTAAGCCTGTTCCTGGTGGG |