| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.036689 |
| Chromosome: | chromosome 9 |
| Location: | 265739 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386758 | ALSL1,ALS1 | (1 of 1) K01652 - acetolactate synthase I/II/III large subunit (E2.2.1.6L, ilvB, ilvG, ilvI); Acetolactate synthase, large subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGAGAAGGTCAGCCGGCGAACGTAGCCAGTAAGGAAGCAGGCGCACTG |
| Internal bar code: | GATACTTTTGCTGGGCCTGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1601 |
| LEAP-Seq percent confirming: | 85.3659 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTAATTCTGCTGGCTTGCC |
| Suggested primer 2: | AGCACCCCTAGCACCTCATA |