| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.036695 |
| Chromosome: | chromosome 6 |
| Location: | 7830916 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g303400 | ADF3,FAP16 | (1 of 1) K18597 - echinoderm microtubule-associated protein-like 5 (EML5); necessary for axonemal severing | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCGGCTCCGCCACCCAATGTACCTCGCTTACCGCAAGCCTGAATGCA |
| Internal bar code: | GATAGATACGGTTGTATAAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 179 |
| LEAP-Seq percent confirming: | 58.3333 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACCTCCCATCCCTCCAC |
| Suggested primer 2: | AACAAACGCACGACCTTGTG |