Insertion junction: CLIP2.036721_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.036721
Chromosome:chromosome 17
Location:3301346
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g722250 RRM14 Putative rRNA (guanine-N2-)-methyltransferase; (1 of 1) 2.1.1.173//2.1.1.264 - 23S rRNA (guanine(2445)-N(2))-methyltransferase // 23S rRNA (guanine(2069)-N(7))-methyltransferase / 23S rRNA m(7)G(2069) methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACACACGCTCATCGCCTGCGCCACACCGCAGCTGTACGACATGGTGTA
Internal bar code:ACTTCTCCCGCAGTTTCGTCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:4077
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:ACACAGAGGGCCAAACCAAA
Suggested primer 2:CCCTCACTTTGGCCGATTCT