| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.036724 |
| Chromosome: | chromosome 6 |
| Location: | 7348646 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299850 | HLM10 | (1 of 2) K11426 - SET and MYND domain-containing protein (SMYD); Histone-lysine N-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGCTGGCGAGGCGGCCGCTGGTACCGGTACCGGTTATAGTGTGGGCTG |
| Internal bar code: | ATAATCATAGTTTGCAGCAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1211 |
| LEAP-Seq percent confirming: | 12.5 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACTGTACATGAGCGCACA |
| Suggested primer 2: | GAGTGCCTGACTACAACGCT |