| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.036725 |
| Chromosome: | chromosome 4 |
| Location: | 1990579 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219000 | (1 of 2) PTHR10887//PTHR10887:SF333//PTHR13779//PTHR13779:SF20 - DNA2/NAM7 HELICASE FAMILY // SUBFAMILY NOT NAMED // HOLLIDAY JUNCTION DNA HELICASE RUVB-RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTTGCGGGGTCAGGGCAGCGCGGTCACAGCGGCAGGCGGTAGTGATT |
| Internal bar code: | CGAACAAGTGACAGTGGCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3266 |
| LEAP-Seq percent confirming: | 88.3929 |
| LEAP-Seq n confirming: | 99 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 112 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACTACAGCGATGAGGAGC |
| Suggested primer 2: | ACGAGTGAACACGACTCACC |