Insertion junction: CLIP2.036726_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.036726
Chromosome:chromosome 6
Location:1376423
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g259500 CYG2 Adenylate/guanylate cyclase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGGCCTCAGCAACCATCAGTGCGCAAACGGTCGTGGGTGCTGACCGCT
Internal bar code:ACGAGGCAACAGTGGGTCGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:1878
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:TAAGACCTGACGCAAACCCC
Suggested primer 2:GACACGCCCATATCGTTTGC