Insertion junction: CLIP2.036727_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.036727
Chromosome:chromosome 12
Location:3445972
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g497450 FAP412 (1 of 21) IPR001611//IPR003591 - Leucine-rich repeat // Leucine-rich repeat, typical subtype; Flagellar Associated Protein 412 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACATGACGCTGGCTTCACTGCCGGGAGCATCATGATTCATGAGTGCGTG
Internal bar code:GACTTCCGGGCGACAGCGGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:299
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TTACACCTCTTCACGCGGTC
Suggested primer 2:GATCTCCACTGCTGAGGAGC