Insertion junction: CLIP2.036735_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.036735
Chromosome:chromosome 12
Location:5982746
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g534151 EEP9 (1 of 3) PTHR22748:SF4 - DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE 2; endonuclease/exonuclease/phosphatase family protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTGCCCTGCAGCGCTAGCAACCCCATGCCGCGGCGGTCCATAAGACAT
Internal bar code:CGAACTCGAATTGGATCGGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2734
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCAATGGAAGCCGCCTAT
Suggested primer 2:ACAAGCGCCAAAGTCTATGC