Insertion junction: CLIP2.036746_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.036746
Chromosome:chromosome 1
Location:2105118
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g011750 (1 of 1) PTHR31353:SF1 - PROTEIN TAG-322 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCAAAACTCTTTTTCATGACACGCTCTGTCACCTGCACACGCCACGA
Internal bar code:GGACGGTGATTGTCCAGTAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:89
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTCCTCCTCCTCCTTCT
Suggested primer 2:CGTTGCTGGTGTCACAAAGG