Insertion junction: CLIP2.036764_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.036764
Chromosome:chromosome 16
Location:7692580
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g686173 (1 of 1) PTHR11071:SF276 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTCGATGTTCTTCTTTATCTCCCGCAAACTGGTTCCCGCACTCGGTTTG
Internal bar code:ATGGCCTCGTCCTGCAGTTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:4085
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:91
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:91

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCTTGGAACCGTGAAACC
Suggested primer 2:GTGGTCCTCGAACAGCTCAA