Insertion junction: CLIP2.036776_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.036776
Chromosome:chromosome 16
Location:4063013
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g690450 FAP266 Flagellar Associated Protein 266; (1 of 1) PTHR23084//PTHR23084:SF143 - PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTATCGAGGACACACGCGCCTCCTGCCATCCCCACCCACAGCCCACCCCA
Internal bar code:ATACATAAGGACCTGATGACAT

Confirmation - LEAP-Seq

LEAP-Seq distance:58
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTCAACGACCACAAACCTGC
Suggested primer 2:CCATGGTCATGCTGAGGTGT