| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.036789 |
| Chromosome: | chromosome 2 |
| Location: | 1572989 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g084800 | SPO11A | (1 of 1) K10878 - meiotic recombination protein SPO11 (SPO11); meoisis-related DNA topoisomerase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGTCGATTGTCGACTTTCGACCTATCCAAGTATGGGGCTGGAACTTC |
| Internal bar code: | GAGTGTTCCCATTCACTTAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 513 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGATGATGCAGGGGTAGGT |
| Suggested primer 2: | ACTACCGTAGTGGAGCGTCT |