Insertion junction: CLIP2.036825_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.036825
Chromosome:chromosome 7
Location:440155
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g315250 CSG5 Chlamydomonas specific family G protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTTGCCGCGCGGCACGAGGAGAAGACCCTGAGCCGGCGCGTACTGGAG
Internal bar code:AAGGGCGGCTGTAGGAGGCGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1917
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:61
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCTACACACACACGGCTG
Suggested primer 2:AGTCCTGAGCTTGTTCTGCC