| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.036831 |
| Chromosome: | chromosome 3 |
| Location: | 6570343 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194500 | (1 of 2) IPR000719//IPR011009//IPR013221 - Protein kinase domain // Protein kinase-like domain // Mur ligase, central | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGAAACACTTCGACACTTTCGTAAACCGGTCTCGGAGACTCGGCCACA |
| Internal bar code: | ATCCTAATTATTTCGCTAGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5339 |
| LEAP-Seq percent confirming: | 23.6842 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 116 |
| LEAP-Seq n unique pos: | 152 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGTTAGCGAAGGCGAAG |
| Suggested primer 2: | ACGGGTAGCTCTCTGGCTTA |