| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.036832 |
| Chromosome: | chromosome 2 |
| Location: | 910154 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g079000 | POB21 | Proteome of basal body 21 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGACTGATGGGCGCGGGTGGCGGAGGGCGGCGATAGCGGGCAGCACGCT |
| Internal bar code: | ATTTAATGGGTGAAGATAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6200 |
| LEAP-Seq percent confirming: | 17.4603 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTGGTCTCATAGTGCA |
| Suggested primer 2: | ATTTGCACGATGAGGAGCCA |