| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.036855 |
| Chromosome: | chromosome 3 |
| Location: | 4582322 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176450 | (1 of 17) IPR029069 - HotDog domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGAGTATCAGGAAGGCCCGTCAGTTGCGGTGCAGAGCGTGACCAGTTG |
| Internal bar code: | CGGCTATCAATATCGCAATTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 657 |
| LEAP-Seq percent confirming: | 76.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATGAGCTGCTGCGGATTC |
| Suggested primer 2: | GGCCCGCTGCAATGTTTTTA |