Insertion junction: CLIP2.036855_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.036855
Chromosome:chromosome 3
Location:4582324
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g176450 (1 of 17) IPR029069 - HotDog domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATACTCTGTCCTGGCAGGTGGCCTGACAGGTGGCCTGACAGGTGCCATTC
Internal bar code:CGGCTATCAATATCGCAATTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1301
LEAP-Seq percent confirming:96.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GGCCCGCTGCAATGTTTTTA
Suggested primer 2:CAATGAGCTGCTGCGGATTC