Insertion junction: CLIP2.036865_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.036865
Chromosome:chromosome 3
Location:3740064
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g169200 (1 of 1) 2.7.7.5 - Sulfate adenylyltransferase (ADP) / ADP-sulfurylase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCAGGAGCACCTTGGCGCGACGAAACCACCTTCACAGCGCGAGGACCG
Internal bar code:TTGCTTATGTCTTCCAAGTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2173
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:TGCAGAGCACAACAACATGC
Suggested primer 2:CCACTACTGTTGACCAGCGT