| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.036880 |
| Chromosome: | chromosome 4 |
| Location: | 3107033 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225750 | (1 of 1) K18550 - IMP and pyridine-specific 5'-nucleotidase (ISN1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCAGAGCGAAAGCTTCAGGATTCCCAAACTCGAGCCCCACTCCTTAC |
| Internal bar code: | CTTAGAGGACAAGCAACAATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1217 |
| LEAP-Seq percent confirming: | 75.2688 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGGAAGGCGACAGGGAA |
| Suggested primer 2: | CACGTTGCTCCTCATCAGGT |