| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.036885 |
| Chromosome: | chromosome 17 |
| Location: | 226280 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g697350 | (1 of 1) K12861 - pre-mRNA-splicing factor SPF27 (BCAS2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCAGTATATCGCACGCCAGGGCCTCCGCGCGTGTGTCGGCGTGCCCCG |
| Internal bar code: | GGGCTCGGGTGGACGATGTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2265 |
| LEAP-Seq percent confirming: | 55.5556 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGCCTCAGGTTAGGTTT |
| Suggested primer 2: | ATTGCATGGTCAGCAGGGAA |