| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.036892 |
| Chromosome: | chromosome 5 |
| Location: | 2384812 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238374 | (1 of 1) K11975 - E3 ubiquitin-protein ligase RNF144 [EC:6.3.2.19] (RNF144) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTGTAAAGTGAAGGCGGGGGAGGGGGGTGTAGCCCTGCATGAGAAAC |
| Internal bar code: | AAATACACACATCAATTTTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 57 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCCCCGCGTGTCATATC |
| Suggested primer 2: | ACACCTGCTGTCTACTTCGC |