| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.036909 |
| Chromosome: | chromosome 3 |
| Location: | 5320742 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182800 | AGT2 | Alanine-glyoxylate transaminase; (1 of 1) K00827 - alanine-glyoxylate transaminase / (R)-3-amino-2-methylpropionate-pyruvate transaminase (AGXT2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACCCCGTGTGCTCGGCTGCCGGGCGCGCGGTGCTGCGGGTTGTGGACA |
| Internal bar code: | GTACGATGTTACCAACTAAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1182 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGCCCATGATGCCTCCAA |
| Suggested primer 2: | TGTACTTGGGCTGAATGCGT |