| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.036923 |
| Chromosome: | chromosome 13 |
| Location: | 3887609 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g589700 | VPS20 | Subunit of ESCRT-III complex; (1 of 1) K12195 - charged multivesicular body protein 6 (CHMP6, VPS20) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGCATGTGTAGGGTCCGTGTGCTTTCTGGTTGCAGCCCAGTTGCATCA |
| Internal bar code: | TGAAACGTATAGTTACATTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2492 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCATCCACCACCACCTCC |
| Suggested primer 2: | CCAACGGACACAAACGGTTC |