Insertion junction: CLIP2.036932_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.036932
Chromosome:chromosome 16
Location:7492741
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g682026 (1 of 781) IPR000104 - Antifreeze protein, type I CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGAGAGTGCAGATGTGACGGCTGATGAGGATGAGGTGCCGCCGCAAGAG
Internal bar code:GTCCGTTATGCTTTTAGCCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2165
LEAP-Seq percent confirming:16.6667
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AATGCTGTTGGCTGCTGTTG
Suggested primer 2:CGATGTCATTGCAGCACGAG