| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.036953 |
| Chromosome: | chromosome 6 |
| Location: | 2721854 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g270900 | SUV3 | Mitochondrial DEAD-box DNA/RNA helicase; (1 of 1) K17675 - ATP-dependent RNA helicase SUPV3L1/SUV3 [EC:3.6.4.13] (SUPV3L1, SUV3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGATTGCGTCACTAGCCACCAGGATCCGAGAGCCCCTGCCACCGCTA |
| Internal bar code: | GGTATTACGTGTTGCACAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3229 |
| LEAP-Seq percent confirming: | 92.1569 |
| LEAP-Seq n confirming: | 94 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 102 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACGGTGTACTGTTGTGCA |
| Suggested primer 2: | TAGGACAGGACTGAGGAGGC |