| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.036962 |
| Chromosome: | chromosome 4 |
| Location: | 2493881 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g222050 | (1 of 3) K16489 - protein SFI1 (SFI1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAATTTGTTTCTGGGCCCAGGCGGCGGTGCATGAAGATTGAGGCGCCG |
| Internal bar code: | GGTATTGTCACTGTACTAATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 751 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTAAACACAGGAGGGGC |
| Suggested primer 2: | TGGGCTGGTATTGACAACCC |