| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.036978 |
| Chromosome: | chromosome 3 |
| Location: | 6479341 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193600 | (1 of 4) 2.7.11.13 - Protein kinase C / PKC | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCCCGGCACCGTCCACGTCCGACAGAGTCACGTGGCCTGGAGGTGCG |
| Internal bar code: | CCAATTAATGTAGGGCTTCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1509 |
| LEAP-Seq percent confirming: | 95.6522 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCCAATGCTTTCCCTACT |
| Suggested primer 2: | ACGTCCGTATACGCATTCCC |