| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.036983 |
| Chromosome: | chromosome 1 |
| Location: | 5056131 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035200 | (1 of 13) 2.3.1.225 - Protein S-acyltransferase / S-protein acyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGATAAGTGGGCGGTACGCGGCTCAGGAGGCGATACGCCGTTCAAGTAT |
| Internal bar code: | CCTTTGGTACCGCCAGGGAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1311 |
| LEAP-Seq percent confirming: | 55.814 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACATGCAACAGCAAGGAA |
| Suggested primer 2: | CATCTCCTGTCCCGGCATAC |