Insertion junction: CLIP2.037024_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.037024
Chromosome:chromosome 1
Location:4226839
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g028550 OTU1 (1 of 1) PTHR12419:SF10 - PROTEIN OTUB-3, ISOFORM B; OTU-like cysteine protease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCTGTGAGTGTCGTCACACAACCGGGCGCCATCAGGTGAGGCCCAGAT
Internal bar code:AGTTCCGAAGATCTATATCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1222
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCTTGCCAAAAGGGTTTA
Suggested primer 2:AGACTTCCCCGATCTCTGCT