Insertion junction: CLIP2.037026_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.037026
Chromosome:chromosome 9
Location:2161399
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g395600 OBGC,OBGC1 Factor possibly involved in assembly of the ribosomal 50S subunit; (1 of 1) PTHR11702:SF3 - GTP-BINDING PROTEIN 10 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGCCCAGCGCCGCAGCTTCTGGGTGCGCGACGGCCACGGCATCAACCA
Internal bar code:ACACATTGATCGGTCTGGGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:2487
LEAP-Seq percent confirming:98.913
LEAP-Seq n confirming:91
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:92

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTAGGTCCCAGGACATGA
Suggested primer 2:TAACGTCATGACACAGCCCC