| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.037038 |
| Chromosome: | chromosome 9 |
| Location: | 6424420 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g414900 | ELG33,ELG41,ELG32 | (1 of 2) PTHR11062//PTHR11062:SF89 - EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED // SUBFAMILY NOT NAMED; Exostosin-like glycosyltransferase 41 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATGCGTTCGGAGGGCCACAAGCATATGGTTAGCATTCTTTCTACAGAG |
| Internal bar code: | CATCCGTGTTGATGTTGGTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4223 |
| LEAP-Seq percent confirming: | 98.75 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATGGGCGTTACACGTTCA |
| Suggested primer 2: | AGCTGGAATTGAGAGTGCCC |