Insertion junction: CLIP2.037050_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.037050
Chromosome:chromosome 10
Location:2713012
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g437800 (1 of 3) IPR000104//IPR001054//IPR006059//IPR029787 - Antifreeze protein, type I // Adenylyl cyclase class-3/4/guanylyl cyclase // Solute-binding family 1 // Nucleotide cyclase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAGTAGGGGTGCAGGGAGGGCGGGGAGTGCAATGCGTCAATGCAATCA
Internal bar code:GGTACTACGTCTCGTAGTAACC

Confirmation - LEAP-Seq

LEAP-Seq distance:2527
LEAP-Seq percent confirming:96.4286
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:TGGAACCCGCAGCTAACATT
Suggested primer 2:ACCACGTATCTGGCTGTGTG