| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.037067 |
| Chromosome: | chromosome 14 |
| Location: | 2524539 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g624550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGGGCACAATACTTTAGTGATGCGGGTGTCACTCCTTGCTCGGTTGCA |
| Internal bar code: | GTTAAGGTGGACCGTCCTCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6058 |
| LEAP-Seq percent confirming: | 85.0 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGAGGTTTGGCCATGGG |
| Suggested primer 2: | GCACATACTCCCGCGATACA |