| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.037068 |
| Chromosome: | chromosome 2 |
| Location: | 4491391 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g800228 | (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGGCTTGCGCCACCTGTGTGGGATGTGGTGTGTCTCGCTGCTATGTCT |
| Internal bar code: | AGTCAGATCAGATGAGAACTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5072 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 110 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAAAAATGGCCGCTTTGA |
| Suggested primer 2: | TTGGTGAAGCGTGTGTGACT |