| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.037083 |
| Chromosome: | chromosome 16 |
| Location: | 1648222 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTCCCTAACTCCCTACCTCCCACCACCTCCCTACCTCCCTACCTCCCA |
| Internal bar code: | AAGATTCCTGCTGTACCGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3708 |
| LEAP-Seq percent confirming: | 48.0769 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCACACCAATTTCCAACT |
| Suggested primer 2: | CCTGATCGACCACTACGAGC |