| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.037084 |
| Chromosome: | chromosome 6 |
| Location: | 4282541 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278257 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGGCTTGGCGCAGCCTCAGGGGCCTCCGGCGCCTGGGCAGCAGCAGCA |
| Internal bar code: | TAGAATATAGATCAGTTGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2773 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTAGACGCGCCTGTTTCCT |
| Suggested primer 2: | GCTGCTTAGTTCGTTTGCGA |