| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.037090 |
| Chromosome: | chromosome 6 |
| Location: | 8433718 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g308300 | RRM6 | (1 of 2) IPR002877//IPR029063 - Ribosomal RNA methyltransferase FtsJ domain // S-adenosyl-L-methionine-dependent methyltransferase; Putative ribosomal RNA methyltransferase of the RrmJ/FtsJ family | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATAGGCATGGGTCCGCCGTAGCCGGCCGCAGGCACCGGCATGGGCATCG |
| Internal bar code: | GTCGAAACCAGGAATCTGTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4942 |
| LEAP-Seq percent confirming: | 90.6433 |
| LEAP-Seq n confirming: | 155 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 171 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTAGACGTTCGACTGGCA |
| Suggested primer 2: | GGGAGCGTAAGACCGATGAG |