| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.037131 |
| Chromosome: | chromosome 16 |
| Location: | 6322367 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675850 | (1 of 1) K11729 - acyl-CoA dehydrogenase family member 10 (ACAD10) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGTGCACTAGGTGGGTGTTGGGTGGGGTGTGGAGTGCAGGTGGCGGC |
| Internal bar code: | TTGTCACAAGTGAAACTTGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 231 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGAGCGAGCAAGAGAGAC |
| Suggested primer 2: | TCACATCACCCTGGAATGCC |