Insertion junction: CLIP2.037162_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.037162
Chromosome:chromosome 13
Location:483877
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g565050 (1 of 11) PF07173 - Protein of unknown function (DUF1399) (DUF1399) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGAACTGACACAGATACCCCCGGCGTCACCATCAACATCACCGCTGCTG
Internal bar code:TGTCAACTCTGCTCGAAGGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:150
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGTCTTCCCCAGCATCCG
Suggested primer 2:CTGCTTCTCTTCCCTTGGGG