| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.037182 |
| Chromosome: | chromosome 4 |
| Location: | 1757699 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g211950 | ELG32 | Exostosin-like glycosyltransferase 32; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGAAGCCAAAACAGTCCAGCTGCAAAACAGCAGTTTGACATTTTCTC |
| Internal bar code: | TAGCGCATTCGACTGGAGTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2284 |
| LEAP-Seq percent confirming: | 91.3043 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCCTCAGCTCTGTTCAAT |
| Suggested primer 2: | GCTTGGGCTTGTACCTGGAT |