Insertion junction: CLIP2.037184_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.037184
Chromosome:chromosome 17
Location:5848580
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g741500 KIL7 Putative kinesin-like motor protein; (1 of 1) 3.4.21.72//3.6.4.4 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease // Plus-end-directed kinesin ATPase / Kinesin intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCTGAGCACGGGGTGGCGCTGCGTACAGCTTGCACGAGCGATACGACGA
Internal bar code:TATGGACTATCGTGATTCTCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:4414
LEAP-Seq percent confirming:46.3415
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:44
LEAP-Seq n unique pos:82

Suggested primers for confirmation by PCR

Suggested primer 1:CTACGGTGTTGATGGCCACT
Suggested primer 2:GCATCTTCAGCCTTGTGCAT